THE ROLE OF STRESS-KINASE p38 IN CELL CYCLE BLOCKING DELAY AFTER OSMOTIC STRESS
A. R. Goloudina, 1 V. A. Pospelov
Institite of Cytology RAS, St. Petersburg, Russia;
1 e-mail: nastena18@mail333.com
Alteration in osmotic conditions leads to activation of some defensive mechanisms resulting in blocking the cell cycle
progression. One of stress kinases, activated after osmotic stress, is p38 MAPK. In the present study the role of p38 both in intra-S-phase
and G2/M checkpoint is shown. Besides, p38-dependent degradation of Cdc25A phosphatase after osmotic stress is
demonstrated. Expression of stable form of Cdc25A results in a partial abrogation of G2/M block of the cell cycle, but has
no effect on DNA synthesis arrest.
Key words: osmotic stress, checkpoint, p38 MAPK, Cdc25A, HeLa cells
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