ADHESION AND INTEGRIN EXPRESSION BY IN VITRO MODIFIED ACUTE MYELOID LEUKAEMIA
BLASTS
E. Yu. Sadovnikova, 1 * D. A. Svinareva, 1
E. N. Parovichnikova, 1 E. L. Semikina, 2 E. A. Kopiltsova, 2 N. A. Torubarova, 2
V. M. Belkin, 1 V. G. Savchenko 1
1 National Research Centre for Haemotology RAMS, and 2
Child Health Center RAMS, Moscow, Russia;
* e-mail: sadovnikova@blood.ru
As shown elsewhere, cultured acute myeloid leukaemia blasts acquire certain characteristics of dendritic cells upon
stimulation with cytokines and calcium ionophore. The ability of leukamia-derived dendritic-like cells to express immune costimulatory
molecules and dendritic cell marker CD83 has been extensively investigated. Although migratory capacity is a major attribute of dendritic
cells, the ability of in vitro modified blasts for adhesion, chemotaxis and homing remain elusive. In the present paper, we show that after
stimulation with calcium ionophore acute myeloid leukaemia blasts as well as normal dendritic cell precursors demonstrate increased
capacity of binding fibronectin and denatured collagen. The expression pattern of integrins on dendritic-like leu-kaemic cells in general
closely resembles that of monocyte-derived dendritic cells, however, variation in cell properties isolated from blood of individual patients are
observed.
Back
Contents
Main
|
