DNA QUANTIFICATION IN NUCLEI OF CULTIVATED MUSHROOM WITH DAPI STAINING
E. V. Pancheva, V. N. Volkova, O. V. Kamzolkina
M. V. Lomonosov Moscow State University, Faculty of Biology, Mycology and Algology Department, Russia;
e-mail: agaricus@yandex.ru
Agaricus bisporus (Lange) Imbach is actively cultivated amphithallic basidiomycete, in which various strains
are primary homothallic, heterothallic or secondary homothallic. Countings of relative nuclear DNA con-tence by means of DAPI stain and
its comparison in different strains can help to understand the mushroom's life cycle features. The authors for the first time observed
change of nuclear phases in basidia of A. bisporus strains with different types of life cycle and revealed that DNA contence in diploid
nuclei is about 1.3 times higher than in haploid ones. The method is highly sensitive and can be used for quantitative measurings of
nuclear DNA even in objects with nuclei of about 1 mkm in diameter.
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