EFFECT OF PROGESTERONE ON THEOPHYLLINE AND PROLACTIN STIMULATED Ca2+ EXIT
FROM INTRACELLULAR STORES OF PIG OOCYTES
V. Yu. Denisenko, 1 T. I. Kuzmina
All-Russian Research Institute of Farm Animal Genetics and Breeding RAAS, Pushkin, St. Petersburg,
Russia;
1 e-mail: vitald@fromru.com
Effect of progesterone on theophylline and prolactin stimulated Ca2+ exit from intracellular stores of pig
oocytes was investigated using a fluorescent dye chlortetracycline. It is shown that in progesterone treated oocytes prolactin in concentration
50 ng/ml inhibits Ca2+ exit from intracellular stores of pig oocytes. Theophylline exerts the effect on prolactin Ca2+
exit from intracelular stores of pig oocytes. Employment of protein kinase C inhibitor cancelled inhibitory effect of prolactin and theophylline
on Ca2+ exit from intracellular stores of pig oocytes. Ca2+ exit from intracellular stores of pig oocytes caused a
joint influence of prolactin and GDP, and that of theophylline and GTP. The influence of protein kinase C inhibitor cancelled the stimulating
effect of prolactin and GDP on Ca2+ exit from intracellular stores of pig oocytes also did not render any influence on the action
of theophylline and GTP. These data suggest the influence of progesterone on theophylline and prolactin stimulated Ca2+
exit from intracellular stores of pig oocytes.
Key words: progesterone, protein kinase C, theophylline, prolactin, calcium, pig oocytes
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