THE FORMATION AND MORPHOFUNCTIONAL CHARACTERIZATION OF THE OSTEOBLASTIC PHENOTYPE IN CELL
CULTURES IN VITRO
R. V. Deev, 1 N. S. Nikolaenko, 2, * N. V. Tsupkina, 2 V. G. Gololobov, 1
I. L. Patokin, 3 G. P. Pinaev 2
1 Russian Military Medical Academy, St. Petersburg, Russia,
2 Institute of Cytology RAS, St. Petersburg, Russia and 3 State Research Institute of Highly Pure Biorcagcnts,
St. Petersburg, Russia;
* e-mail: nani@mail.cytspb.rssi.ru
The objective of this research was to study osteogenic properties of cultured rabbit bone marrow stromal cells,
newborn rat cranium bone cells and rat osteocarcoma ROS 17-2/8 cells. For this purpose cytochemical reaction for alkaline phosphatase
was performed by the Lowry method, mineral deposition was assessed by staining of the cultures after von Kossa. Cranium bone cells
were shown to synthesize alkaline phosphatase (34±7 nmol/min/106 cells), the matrix mineralization being found.
Bone marrow stromal cells displayed a lower activity alkaline phosphatase level than did cranium bone cells
(4±0.6 nmol/min/106 cells). However, cell cultivation in the presence of dexamethasone in the medium (10-8 M)
induced a higher activity of alkaline phosphatase (9±1 nmol/min/ 106 cells), mineralization of the extracellular matrix being
the case. The highest level of alkaline phosphatase activity was found for ROS 17-2/8 cells (60±12 nmol/min/106 cells)
but no matrix mineralization was determined. According to these data, matrix calcification and formation of bone-like nodules are the most
important properties of osteoblastic differentiation in vitro.
Key words: osteoblasts, osteoblastic phenotype, osteogenesis in vitro, differentiation,
cell cultures
Back
Contents
Main
|
