LOCALIZATION OF ATPase ACTIVITY, RESPIRATION AND ULTRASTRUCTURE OF WHEAT ROOT CELLS WITH
MODULATED ION CONDUCTIVITY OF PLASMA MEMBRANE
F. A. Chernyshova, V. Y. Alekseeva, 1 O. O. Polygalova, L. K. Gordon
Institute of Biochemistry and Biophysics, Research Center RAS, Kazan';
1 e-mail: aleksecva@mail.knc.ru
Changes in the localization of ATPase activity, respiration and ultrastructure of wheat root cells with modulated ion
conductivity of plasma membrane were studied. A 2 h treatment of excised root with valinomycin (20 μM), N,N-dicyclohexylcarbodiimid
(100 μM), gramicidin S (20 μM) and chlorpromazine (100 μM) caused an increased loss of potassium by cells, a decreased respiration
and changes in the localization of ATPase activity and in cell ultrastructure. Differences in the observed changes may be conditioned by
different mechanisms of action of the membrane active compounds used. It is concluded that changes in the localization of ATPase
activity and ultrastructure may indicate some early specific responses of root cells, whereas the increase in the ion conductivity and
decrease in respiration under disruption of ion homeostasis caused by membrane active compounds indicate unspecific responses
of cells.
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