MESENCHYMAL STEM CELL TRANSPLANTATION FOR MYOCARDIAL REPARATION OF RAT EXPERIMENTAL HEART FAILURE
P. V. Kruglyakov,1 I. B. Sokolova,1 Kh. K. Amineva,2
N. N. Nekrasova,1 S. V. Viide,1 N. N. Cherednichenko,1 A. Yu. Zarytskii,3
E. N. Semernin,3 T. V. Kislyakova, 1 D. G. Polyntsev 1
1 Trans-Technologies Ltd., 2 Municipal Hospital N 23, and
3 St. Petersburg State Medical University, St. Petersburg, Russia
Mesenchymal stern cells (MSC) are resident pluripotent cells of bone marrow stroma. MSC have the ability to
differentiate into osteoblasts, chondroblasts and adipocytes, neurons, glia and also into cardiomyocytes. The problem of MSC use in cell
therapy of various diseases and in myocardial infarction therapy is widely discussed at present. The experiments were carried out on the
inbred line Wistar-Kyoto rats. Myocardial experimental infarction (EI) was induced by left descending coronary artery ligation. MSC were
isolated from bone marrow, cultivated in vitro and injected into the tail vein on the day of experimental infarction operation. It was
shown that the structure of injured myocardium in experimental group significantly differed from that in control group. MSC
transplantation led to inflammatory process acceleration and to increased angiogenesis in the damaged myocardium; also, live
cardiomyocyte layers were detected in the scar. As a result, ventricular dilatation and overload of the border zone of infarct region
decreased, no features of infarction relapse were shown in the border zone.
Key words: mesenchymal stem cells, capillaries, cardiomyogenic, differentiation, ventricular
dilatation
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