PLANT RESISTANCE INDUCTORS AND ACTIVE FORMS OF OXYGEN. II. THE INFLUENCE OF CHITOOLIGOSACCHARIDES ON THE PRODUCTION OF
HYDROGEN PEROXIDE WITH THE INVOLVEMENT OF OXALATE OXIDASE IN COMMON CULTURES OF WHEAT CALLUSES AND BUNT
PATHOGEN
N. B. Troshina, I. V. Maksimov, L. G. Yarullina, O. B. Surina, E. A. Cherepanova
Institute of Biochemistry and Genetics, Ufa Research Center, RAS, Russia;
e-mail: phyto@a irb.ru
We have studied the influence of various concentrations of chitooligosaccharides (CO) on hydrogen peroxi-de (Í2Î2) generation, with
the involvement of oxalate oxidase (OO), in rhizoids and in zones of hard brand pathogen penetration, as well as on oxalate oxidase activity in wheat calluses. In the control group,
diaminobenzidin (DAB)-staining was typical of 30 % of peripheral rhizoid cells, which provided their resistance during infection. In the zone of fungus penetration, the appearance of
DAB-stained parenchyma-like cells was observed. Simultaneously, the activity OO cytoplasmic fraction increased, whereas OO activity in its ion-bound fraction was suppressed.
Low concentrations of medication induced rhizoid formation, increased the number of DAB-stained cells in the pathogen p netration zone, and induced OO activity in cytoplasmic
and ionically cell wall-bound fractions. On the contrary, a high concentration of CO (100 mg/ml) suppressed rhizoid formation, OO activity and the fungus growth. The discovered
correlation between enzymatic activation under CO influence, the high level of protective response during infection, and the intensity of rhizoid formation may suggest the community
of protective and morphogenetic mechanisms of reactions in plant cells in respect to hydrogen peroxide production.
Key words: callus, rhizoid, bunt pathogen, chitooligosaccharides (CO), hydrogen peroxide
(Í2Î2), diaminobenzidine, oxalate oxidase (OO)
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