PLANT RESISTANCE INDUCTORS AND ACTIVE FORMS OF OXYGEN. I. THE INFLUENCE OF SALICYLIC ACID ON HYDROGEN PEROXIDE PRODUCTION
IN COMMON CULTURES OF WHEAT CALLUS AND BUNT PATHOGEN
N. B. Troshina, I. V. Maksimov, L. G. Yarullina, O. B. Surina, E. A. Cherepanova
Institute of Biochemistry and Genetics, Ufa Research Center, RAS, Russia;
e-mail: phyto@a irb.ru
The influence of salicylic acid (SA) on cell resistance to bunt pathogen in wheat calluses has been studied. Cell staining by diaminobenzidin substratum
(DAB-cells) was conditioned by hydrogen peroxide (Í2Î2) generation with the involvement of oxalate oxidase (OO). In the control group, DAB-staining
was typical only of rhizoid cells (up to 50 %). The infection caused no significant increase in the nun ber of such cells; however, in the zone of fungus penetration parenchyma-like
DAB-stained cells were observec (up to 24 %). Under the influence of SA, the number of DAB-stained cells did not change, but increased in the zone of fungus penetration
(up to 36 %). Besides, SA increased OO activity and accelerated proembyogenic complex formation in the calluses, which, unlike rhizoids, were not sensitive to the phytopathogen.
The infection caused an increase of OO activity in the cytoplasm and in an intercellular fraction, and an inhibition of an extracellular fraction of the enzyme.
Key words: wheat, callus, cell resistance, oxalate oxidase, hytrogen peroxide, bunt pathogen,
salicylic acid (SA), DAB (diaminobenzidin)-stained cell (DAB-cell)
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