IMMUNOCYTOCHEMICAL LOCALIZATION OF VASOPRESSIN AT ITS ABSORPTION BY CELLS OF RAT SMALL
INTESTINE
E. V. Seliverstova,1 E. I. Shakhmatova,1 Ya. Yu. Komissarchik,1 N. P. Prutskova,1
E. S. Snigirevskaya,2 Yu. V. Natochin 1
1 I. M. Sechenov Institute of Evolutionary Physiology and Bioche nistry RAS, and
2 Institute of Cytology RAS, St. Petersburg, Russia;
e-mail: natochin@iephb.ru
Morpho-physiological characteristics of the transport of cyclic nonapeptide arginine vasopressin (AVP) across the rat intestinal epithelium was studied in
experiments in vitro. A partial absorption of physiologically active AVP was followed when filling the isolated intestinal lumen by hormone solution. By methods of
immunoelectron and immunofluorescence confocal microscopy, using polyclonal anti AVP antibodies, cytoplasmic localization of AVP label was shown in enterocytes.
The AVP label was also observed in the intercellular space in the basal area of epithelium. No label was revealed in the intercellular junctiors, and no predominant label
accumulation was found in any cytoplasmic structures of the epithelial cells. The obtained results are considered as evidence for the transcellular pathway of partial AVP
absorption in rat small intestine.
Back
Contents
Main
|
