ORGANELLE REDISTRIBUTION DURING PK CELL SPREADING IN NORMAL CONDITIONS AND IN THE
PRESENCE OF Na AZIDE
O. P. Kisurina-Evgenieva, G. E. Onishchenko
Department of Cell Biology and Histology, Biological Faculty, Moscow State University, Russia;
e-mail: cytos@mail.ru
Cell detachment from solid substratum results in a changed arrangement of cytoplasmic organelles and, primarily, in disruption of the centrosome and
Golgi complex colocalization. In the course of cell spreading, reor-ganization of intracellular compartments occurs by stages. At first, mitochondria disperse in the cytoplasm
volume, then the centrosome and Golgi complex unite, and finally collapse of intermediate filaments disappears. In the presence of Na azide the establishment of intracellular
compartments during cell spreading proceeds in a similar mannner. However, unlike the normal conditions, here atypical mtracellular compartments appear: some of these
containing lysosomes and lipid inclusions and located in the perinuclear area, and others with extended cisterns of endoplasmic reticulum on the cell periphery. Additionally,
in the presence of Na azide the density of microtubules and intermediate filaments increases. It has been suggested that changes in the cytoskeleton organization lead to an
enhanced cell spreading.
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