IMMORTALIZED CELL LINES FROM MURINE EMBRYOS ARE CHARTERIZED BY PROGRESSIVE
DESTABILIZATION OF THEIR KARYOTYPE STRUCTURE
T. M. Grinchuk,1 N. A. Pugovkina, M. V. Tarunina, E. A. Sorokina, T. N. Ignatova
Institute of Cytology RAS, St. Petersburg;
1 e-mail: gmdmk@mail.cytspb.rssi.ru
The karyotype structure was studied for three cell lines obtained from cells of transgenic murine embryos at early stages
of their establishment. The first line was obtained from a transgenic embryonic explantant containing oncogen v-sis under promoter
MMTV, two other lines originated from cells of transgenic embryos containing oncogen k51. The karyotypic analysis of
G-banded metaphase chromosomes revealed deviations from the normal mouse karyotype as early as by the third passage of cultivation
of independent embryonic cell lines that contained a foreign oncogene in their genome. The repeated analysis that involved 15-22
passages revealed similar abnormalities: variability and progression in chromosome number with the appearance of hyperpolyploid
combinations, and a large number of rearranged chromosomes, both marker and unique ones. It is concluded that introduction of a
foreign oncogene into murine cell genome leads to its enhanced and progressive non-specific destabilization. Oncogen v-sis
produces a more valuable karyotype destabilization than oncogen hSl.
Key words: transgenic embryos, cell lines, immortalization, karyotype, destabilization
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