EFFECT OF NITRIC OXIDE DONOR (NaNO2) ON THE STABILITY OF NON-TRANSFORMED AND MALIGNANT
CELLS TO ULTRAVIOLET AND GAMMA-RADIATION
O. I. Bol'shakova, A. G. Sverdlov, S. I. Timoshenko, N. G. Nikanorova, G. N. Bondarev, S. A. Grachev
St. Petersburg Nuclear Physics Institute RAS, Gatchina, Leningrad District,
Russia
A decreased sensitivity of the Chinese hamster cells (line V-79) to gamma-radiation under the influence of nitric oxide
induction was shown elsewhere. This effect is connected hypothetically with post-radiation reparation of DNA. The investigation of the
nitric oxide donor effect on sensitivity of these to UV-radiation is of interest, because this radiation is an important ecological factor of the
environment. The question of retention of nitric oxide positive effect on UV and gamma-radiation sensitivity in malignant HeLa cells is no
less actual, because these cells significantly differ from normal cells of line V-79. We demonstrated that the donor of nitric oxide enhances
stability of the Chinese hamster cells (line V-79) to UV-radiation, as well as to gamma-radiation independently of the time of cell incubation
with sodium oxide donor before or after irradiation. The inefficiency of nitric oxide as a factor increasing UV-stability of cells was shown for
malignant HeLa cells. A 1 h long incubation of these cells with NO-donor before gamma-irradiation decreased the number of chromosome
aberrations, and conversely, the addition of this agent to the HeLa cell culture after gamma-irradiation did not change the ra-diostability.
It may be inferred that distinctions in behaviour of nitric oxide in cultures of V-79 and HeLa cells using UV-radiation may be explained by
transformation of the latter special features of their damage, and by the following reparation.
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