PARTICIPATION OF WHEAT GERM AGGLUTININ IN REGULATION OF CELL DIVISION IN APICAL ROOT
MERISTEM OF WHEAT SEEDLINGS
M. V. Bezrukova, A. R. Kildibekova, A. M. Aval'baev, F. M. Shakirova
Institute of Biochemistry and Genetics, Ufa Research Centre of the RAS, Ufa,
Russia
A study was made of cell division activity and hormonal status in roots of 4 day old wheat seedling treated with wheat
germ agglutinin (WGA). The revealed stimulating effect of WGA on mitotic index (MI) and cell area in root extension zone was specific for this
lectin, because gliadin, taken as a control protein, caused no changes in growth parameters. Phytolectins (phytohemagglutinin and
concanavalin A) possessing properties of mitogens rendered no such essential influence on cell growth of wheat. Immunoassay has
shown that WGA-treatment leads to accumulation of auxins and cytokinins in roots. This suggest participation of WGA in regulation of MI of
meristem cells in roots of seedlings during their interaction with phytohormones.
Key words: mitotic index, phytohormones, wheat germ agglutinin, Triticum aestivum L.
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