LOCALIZATION OF ACID ORGANELLES IN FROG SKELETAL MUSCLE FIBERS
S. A. Krolenko,* S. Ya. Adamyan, T. N. Belyaeva, T. P. Mozhenok
Institute of Cytology RAS, St. Petersburg;
* e-mail: krolenko@mail.cytspb.rssi.ru
By means of fluorescent and phase-contrast microscopy the distribution of acid membrane organelles in normal and
vacuolated frog skeletal muscle fibers has been studied. The vacuolation of the T-system was produced by loading
and subsequent removal of glycerol (80-110 mM), or it appeared as a result of Zenker's necrosis. Acridine orange
(인) was used as a marker for acid intracellular compartments. 인 accumulated in granules localized near the
nuclear poles (more seldon around the nucleus) and in the intermyofibrillar spaces. Typically the 인 granules make
up short longitudinal chains or regular pairs, where the distances between neighboring granules are short-dated to
sarcomere lengths. Almost all granules emit in red, but about one third of them simultaneously emit in green, which
is characteristic of 인 monomers. In the vicinity of necrotic boundary or under the influence of brefeldin A, a
green component of fluorescence appears in most granules. Treatment with monensin leads to granule disappearance.
Vacuoles accompanying the glycerol treatment or developing of necrosis do not accumulate 인 and exert no effect on
the localization of 인-granules. The nature of cellular organelles accumulating 인 in skeletal muscle fibers is
discussed.
Key words: skeletal muscle, fluorescent microscopy, acridine orange, vacuoles, T-system, necrosis,
acid organelles
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