THE ORIGIN OF NEOINTIMAL CELLS IN THE RAT CAROTID ARTERY AFTER BALLOON ANGIOPLASTY
O. P. Ilyinskaya,1 E. Yu. Kudryashova,1 Yu. G. Antropova,1
N. I. Kalinina,1 M. A. Solomatina,1 A. Bobik,2 E. M. Tararak 1
1 Institute of Experimental Cardiology, Russian Cardiology Research Center, Moscow, and
2 The Baker Medical Research Institute, Melbourne, Australia;
e-mail: cardiocell@cardio.ru
At present the issue of a possible role of circulating stem cells and precursors in pathological vascular wall
remodeling after angioplasty remains unsolved. Therefore the origin of neointimal cells was examined in the rat
carotid artery after balloon angioplasty using morphological and immunocytochemical approaches. It is shown that at
the early stages (1-7 days) after vessel injury acute inflammatory response arises in the arterial wall recruiting
neutrophils, monocytes, macrophages as well as large amounts of low-differentiated blood-derived cells. At the late
stages (10-28 days), at the area of injured intima, a new hyperplastic intima (neointima) is formed, which consists
of cells carrying specific smooth muscle markers - α-actin and smoothelin. The study on cell proliferative
behaviour in the injured vessel wall by bromodeoxyuridine showed that in the process of neointima formation
blood-born rather than resident cells are involved. Probably, early smooth muscle and endothelial precursor cells
penetrate into injured area with blood stream, where they proliferative and differentiate into mature cells.
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