NEONATAL RAT CARDIAC VENTRICULAR CELLS IN CULTURE: DNA SYNTHESIS, ULTRASTRUCTURE AND
LOCALIZATION OF ATRIAL NATRIURETIC PEPTIDE
I. L. Erokhina,* E. G. Semenova, O. I. Emelyanova, M. I. Krylova
Institute of Cytology RAS, St. Petersburg;
* e-mail: heartdev@mail.cytspb.rssi.ru
We determined the optimal conditions suitable for expanding cardiac cells in vitro for their future use in
experimental transplantation into injured myocardium of adult animals. Ventricular cardiac cells were isolated enzymatically
from 2-3 day-old rats and cultured at different cell densities within 5-7 days to 4 weeks. Mixed cultures of muscle and
non-muscle cells were examined by light autoradiography, electron microscopy, and immunogold method. The best results were
obtained at a density of 3 o 105 cells/ml in the medium, consisting of 90% DMEM and 10% fetal calf serum, during 5-7 days of
cultivation. In such cultures myocytes made 62.5 ± 7.9 %. After a 24 h incubation with 3H-thymidine, 22.0 ± 2.2 % of
myocytes were labeled. Muscle cells contact with each other and with non-muscle cells, contain myofibrils, contract and display
atrial natriuretic peptide (ANP)-like immunoreactivity.
Key words: cardiomyocytes, cell culture, DNA synthesis, ultrastructure, neonatal rats, ANP
Back
Contents
Main
|
