METAPHASE CHROMOSOMES OF HUMAN MULTIPLE MYELOMA CELL LINE U-266: KARYOTYPE AND
MORPHO-FUNCTIONAL CHARACTERISTICS OF NUCLEOLAR ORGANIZER REGIONS
V. I. Turilova,1 A. A. Pendina,2 T. D. Smirnova 3
1 Institute of Cytology RAS, 2 D. O. Ott's Institute of Obstetrics and Gynecology RAMS,
and 3 Institute for Influenza Research RAMS, St. Petersburg;
1 e-mail: turilova@mail.cytspb.rssi.ru
Human multiple myeloma (MM) cell lines are widely used to investigate chromosome rearrangements typical for this
disease. However, during cell cultivation both numeral and structural chromosome rearrangements usually take place
in addition to changes of structural and functional status of particular chromosome regions. We investigated
karyotype and morpho-functional status of nucleolar organizer regions (NORs) in human cell line MM U-266.
Cytogenetic analysis (G-banding) showed karyotypic stability and balanced chromosome set in hypodiploid (n
= 44) U-266 cells. We found the presence of rearranged chromosomes, typical for U-266, which retained throughout
many year cell cultivation. At the same time, further chromosome rearrangements were shown, along with a tendency
of cells to polyploidization. Using FISH (rDNA-probe) and AgNOR-staining techniques, we found that only 4 of 8
NORs were Ag-positive (AgNOR), whose dimensions varied from 1 to 3 units of arbitrary scale (u. a. s.). The average
summarized AgNOR size was 7.19 ± 0.03 u. a. s. Peculiarities of the NOR morpho-functional status in U-266 cells
are discussed.
Key words: cell line U-266, karyotype, nonrandom chromosome rearrangements, nucleolar organizer
regions, ribosomal genes, FISH, AgNOR-staining
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