MORPHOLOGICAL CHANGES IN THE KIDNEY AFTER 5/6 NEPHRECTOMY AND LOW-DOSE RADIATION
M. Aunapuu,1 U. Pechter,1 P. Roosar,1 E. Gerskevits,2
M. M. Marjamagi,2 A. Arend,2 I. Kolts,2 M. Ots 3
Clinics of 1 Rehabilitation, 2 Radiology, and 3 Internal Medicine,
Department of Anatomy, University of Tartu, Estonia;
e-mail: mariaun@ut.ee
In the present study, we investigated the effect of low-dose irradiation of experimental nephrectomized rats.
We hypothized that the low-dose irradiation may slow down the development of focal-segmented glomerulosclerosis
(FSGS) after 5/6 nephrectomy. Experiments were performed with 32 male Wistar rats, divided into four groups. The
first group contained only operated animals. Animals in the second and third groups were irradiated on the next
day after operation with 1 and 3 Gy, respectively. The healthy animals made the forth, control group. Attention
was focused on physiological and morphological changes after low-dose (1 and 3 Gy) irradiation. We measured blood
pressure, proteinuria, serum creatinin and cysC. Morphological changes of glomerulus and tubules were studied.
Animals of the first group had significantly thicker glomerular basement membrane, compared to animals of other
groups. The morphological study demonstrated degeneration of the tubular epithelium, tubular atrophy and FSGS.
Besides, it was shown that changes in the third group (3 Gy) were less than in nephrectomized (first group) and 1
Gy (second group). The animals of the third group (3 Gy) had significantly lower proteinuria and FSGS. We conclude
that our hypothesis, suggesting that low-dose irradiation slows down the development of FSGS, was confirmed.
Key words: glomerulosclerosis, rat, kidney, nephrectomy, proteinuria, irradiation
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