INTENSIVE DRYING OF GRIDS AFTER LEAD CITRATE STAINING LOWERS PRECIPITATE
CONTAMINATION ON THE SURFACE OF ULTRATHIN SECTIONS
S. Ye. Khokhlov, A. J. Nevorotin
Central Research Laboratory, St. Petersburg State Medical University after I. P. Pavlov, St. Petersburg;
e-mail: anevorotin@spmu.rssi.ru
Two techniques are proposed to minimize precipitate contamination of ultrathin sections after lead citrate
staining. According to the first one, stained and washed grids are placed, sections downward, on a stack of filter
papers. The second one involves a consecutive washing of grids with distilled water, 96 % ethanol, and hexane.
Both techniques are equally efficient; the former being simpler in processing, while the latter is superior in the
reproducibility of results.
Key words: ultrathin sections, lead citrate staining, contamination, cleaning
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