DELAYED BIOLOGICAL CONSEQUENCES INDUCED BY γ-RADIATION IN HUMAN TUMORIGENIC
DIPLOID LINE SK-UT-1B *
I. S. Smirnova,1 T. K. Yakovleva, N. D. Aksenov
Institute of Cytology RAS, St. Petersburg;
1 e-mail: smir@mail.cytspb.rssi.ru
The progeny of SK-UT-1B cells that survived γ-irradiation with 4 Gy up to the 80th passage was examined.
Descendants of irradiated cells lost p53 transactivation properties. Simultaneously, in the presence of nocodazole
coordination between M and S phases was disrupted. Meanwhile, descendants of irradiated cells maintained the
accurate spindle assembly checkpoint. These data suggest that p53 transactivation function may be required for
coordination of M and S phases, rather than for spindle assembly checkpoint. Since it is known that p53 regulates
both these processes on the basis of data obtained, we suggest that functions of p53 required for coordination of
M and S-phases and for spindle assembly checkpoint are separated. Besides, the data obtained indicate that
radiation-induced chromosomal rearragements are associated with activation of DNA recombination process.
Key words: p53, polyploidy, endoreduplication, delayed radiation-induced genomic instability
* Paper in English.
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