THE EFFECT OF OUABAIN ON SENSORY NEURON NEURITE GROWTH IN ORGANOTYPIC CULTURE
V. A. Pennijajnen,1 B. V. Krylov,1 N. I. Chalisova,1, * I. I. Malevskaya 2
1 The I. P. Pavlov Institute of Physiology RAS, St. Petersburg, and 2 The I.I. Mechnikov State Medical Academy, St Petersburg;
* e-mail: nata@infran.ru
Ouabain, a known inhibitor of Na+, Ê+-ATP, taken in a wide range of concentrations, was investigated in organotypic tissue culture of
dorsal root ganglia cells of 10-11 day old chick embryos. Ouabain inhibited neurite growth in a dose-dependent manner. The Hill coefficient was defined as 1, and the KD
value was estimated as 1 • 10-10w M. At inhibitor concentrations exceeding 1 • 10-9 M, the growth of neurites was totally inhibited. It is assumed that
Na+, Ê+-ATPase may play an important role in regulation of the process of neurite growth in sensory neurones.
Key words: Na+, Ê+-ATPase, tissue culture
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