THE INFLUENCE OF AMMONIUM AND NITRATE OF NUTRIENT MEDIUM
ON ULTRASTRUCTURAL ORGANIZATION AND PHOTOSYNTHESIS OF CALLUS CELLSIN GLYCINE MAX L.
V. G. Ladygin,1 G. A. Semenova,2,* A. P. Smolov 1
1 Institute of Basic Biological Problems RAS, and 2 Institute of Theoretical and
Experimental Biophysics RAS, Pushchino, Moscow Region;
* e-mail: ladygin@issp.serpukhov.su
We studied the influence of exogenic ammonium on the functional activity and ultrastructural organization of
cells of the mixotrophic soybean callus (Glycine max L.). Ammonium available in the nutrient medium
increased the chlorophyll content, accelerating the rate of photosynthetic O2 evolution per unit of
biomass. The presence of ammonium in the medium promoted formation of the protein-synthesizing system, which
manifested itself as increased numbers of ribosomes, and thylakoids of chloroplasts, and higher electron density
of the stroma in mitochondria and cytoplasm of mixotrophic cells. It has been concluded that the use of ammonium
may lead to activation of protein synthesis, thus rising photosynthetic activity and favouring formation and
development of membrane structures in chloroplasts.
Key words: soybean Glycine max L., callus, chloroplast, ultrastructure, mitochondria, nitrate,
ammonium
Back
Contents
Main
