Vol. 45 (2003), ¹ 3, p. 290-297
INTRACELLULAR LOCALIZATION OF XCAP-E IN CONTROL CULTURED XENOPUS CELLS AND INHIBITION OF TRANSCRIPTION AND PROCESSING OF rRNA

E. R. Timirboulatova,1 I. I. Kireyev,1 T. V. Kartavenko,1 P. V. Gulak,2 V. Yu. Polyakov,1 K. LeGuellec, R. E. Uzbekov 1, 3

1 Research Institute of Physico-Chemical Biology, Moscow State University, 2 Institute of Gene Biology RAS, Moscow, and 3 Laboratoire de Biologie et Genetique du Developpement, Rennes Cedex, France;
1 e-mail: al_mira@rambler.ru

The interaction of condensin subunit XCAP-E with various nucleolar subcompartments in XL2 cells was studied. In the interphase cells, XCAP-E was associated with a granular component of nucleoli (as shown by double staining with antibodies against B23) and with small nucleolus-like structures in the nucleoplasm. Inhibition of transcription by actinomycin D does not disrupt interaction of XCAP-E with the granular compartment of segregated nucleoli. Treatment with DRB 5,6-dichloro-1β-ribofuranozide-benzimidazole causes disintegration of nucleolar fibrillar complexes, but does not affect nucleolar localization of XCAP-E. The data suggest that nucleolar association of XCAP-E is independent on the functional state of the nucleolus, and imply a possible role of this protein in rRNA processing and preribosome assembly.

Key words:  XCAP-E, condensing, nucleolar subcompartments


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