ABERRATIONS IN PROMETAPHASE LEADING TO THE FORMATION OF RESTITUTION NUCLEI
E. G. Seryukova, N. V. Dorogova, N. A. Zharkov, N. V. Shamina
Omsk State Agricultural University, Siberian Research Institute of Agriculture, Institute of Cytology and
Genetics, Siberian Branch of RAS, Novosibirsk
The mechanism of restitution nuclei formation in male meoiosis of Triticum-Agropyrum F1 hybrid
and monosomic wheat line Milturum 553 has been studied. Visualization of division spindle with Navashin's routine
fixationle distribution method accompanied with acetocarmine-coloured squashed preparations revealed that a block in
the kinetochore spindle thread formation, i. e. the absence of chomosome association with the division spindle, is
the basic reason of restitution in these genotypes. On this background, the prometap-hase spindle in abnormal
meiosis of these lines demonstrates a number of vivid and inordinate aberrations revealing some regularities of
spindle structure in the normal plant cell.
Key words: restitution nuclei formation, meoiosis, prometaphase
Back
Contents
Main
|
