INDUCTION OF NUCLEAR ENVELOPE FORMATION AROUND INDIVIDUAL CHROMOSOMES ACTED UPON BY
HYPOTONIC SHOCK
S. Yu. Kurchashova,1 V. V. Filimonenko,2, 4 P. V. Gulak,3
I. I. Kireyev,3
V. Yu. Polyakov,1 P. Hozak 2
1 Department of Electron Microscopy, Research Institute of Physico-Chemical Biology after
A. N. Belozersky, Moscow State University,
2 Institute of Experimental Medicine, Prague,
3 Institute of Gene Biology RAS, Moscow, and
4 Institute of Cytology and Genetics, Siberian Branch of RAS, Novosibirsk;
e-mail: svetak99@mail.ru
In the present work we have studied the distribution of some proteins participating in the nuclear envelope
assembly (lamins À/Ñ, Â and LAP2α) in mitotic cells and after hypotonic treatment with 15% Hank's solution.
In untreated cells, these proteins are localized in the nuclei of interphase cells migrate to the cytoplasm during
mitosis. Hypotonic treatment of interphase, prophase and telophase cells does not lead to considerable relocalization
of lamins A/C and B. However, unlike normal mitosis, in prometaphase and metaphase cells their chromosomes acquire
affinity to lamins and LAP2α. Comparative analysis of lamins and LAP2α distribution have revealed that
chromosomes have special sites for binding with different proteins.
Key words: lamins, LAP2α, chromosomes, hypotonic shock
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