DIFFERENTIAL SENSITIVITY OF CD4+ Ò CELL SUBSETS TO CALCIUM IONOPHORE.
PHENOTYPIC CHARACTERISTICS
S. V. Khaidukov,1 I. V. Kholodenko, I. S. Litvinov
The Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow;
1 e-mail: khsv@mail.ibch.ru
The differential sensitivity of peripheral blood (PB) CD4+ Ò lymphocytes to the calcium ionophore
ionomycin was investigated. Effect of ionomycin exerted on Ò cells was time- and dose-dependent. We have shown that
resistant cells belonged to some distinct Ò cell subsets. The resting naive CD4+CD45RA+ Ò cells
showed a little, if any, resistance to ionomycin treatment. The primed CD4+CD45RO+ memory Ò
cells behaved similarly as did ionomycin-resistant (IR) cells. Although IR CD4+ Ò cells had a typical
"memory" phenotype, some quantitative differences were found in expression of CDlla, CD28, CD29, CD62L and CD243
markers between PB CD4+CD45RO+ Ò cells and corresponding IR cells.
Key words: ionomycin, human peripherai blood, CD4+ memory Ò cells
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