THE CONTRIBUTION OF β1 AND β4 INTEGRINS AND 67 kDa LAMININ RECEPTOR
INTO A431 CELL INTERACTION WITH LAMININ ISOFORMS
O. A. Cherepanova,1 N. V. Kalmykova, M. I. Blinova, G. P. Pinaev
Institute of Cytology RAS, St. Petersburg;
1 e-mail: cheer@mailru.com
Laminins, as basal membrane glycoproteins, are ableto stimulate cell adhesion and migration, and to influence
gene expression. The laminin molecule has a set of bioactive sites that interact with different integrin and
nonintegrin receptors, and, as a result, the reaction of the same cell type to different laminin isoforms may be
different. The aim of this study was to determine the contributions of both integrins with β1 and β4
chains and 67 kDa laminin receptor in the interaction of A431 cells with two laminin isoforms: laminin-1 and
laminin-2/4. The obtained data show that integrin a6p4 is more specific for interaction with laminin-2/4 than with
laminin-1 and takes part in the stage of attachment of A431 cells to laminin. 67 kDa receptor promotes cell
spreading on laminin-2/4 and inhibits cell spreading on laminin-1. An assumption was made about the complex
action of receptors for interaction of A431 cells with laminins ("integrin α6β4 - 67 kDa receptors"
complex).
Key words: laminin, integrins, 67 kDa receptors, YIGSR peptide
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