CHANGES IN THE EXPRESSION OF MEMBRANE MARKERS AND IN THE NUMBER OF HUMAN BLOOD
MONONUCLEARS AFTER A SINGLE AND REPEATED (COURSE) ACTION OF VISIBLE AND INFRARED LIGHT AT THERAPEUTIC DOSES
N. A. Zhevago,1 K. A. Samoilova,1, * T. V. Glazanova,2
K. D. Obolenskaya,1 M. F. Balljusek,3 N. Yu. Romanenko 3
1 Institute of Cytology RAS, 2 Russian Institute of Hematology and
Transfusiology, and 3 Hospital of RAS, St. Petersburg;
* e-mail: kirasam@mail.cytspb.rssi.ru
An attempt has been made to prove that the immunomodulating effect of therapeutic doses of polychromatic
visible+infrared polarized (VIP) light at its application to a small body surface area is connected with a
transcutaneous photomodification of a small amount of blood in superficial skin microvessels. For this purpose,
in parallel experiments, using monoclonal antibodies, the membrane phenotype of circulating blood mononuclears was
studied after irradiation of volunteers, of samples of their blood in vitvo, and of a mixture of the
irradiated and non-irradiated autologous blood in a 1 : 10 volume ratio, thereby modeling events in vivo,
when a small amount of the transcutaneously photomodified blood in the vascular bed contacts its main circulating
volume. In this variant of experiment, a great similarity has been established of changes in expression of
mononuclear membrane markers (CD3, CD4, CDS, CD20, CD16, HLA-DR and to a lesser degree of CD25); the ability has
been proven of the photomodified blood to "translate" the light-induced changes to a much higher volume of
non-irradiated blood, which might represent a mechanism of the systemic immunomodulating effect of phototherapy.
Under conditions in vivo and in vitro, the most "reactive" were HLA-DR+, CD20+, CD16+, CD4+, and
0-cells. An increase of the total number of lymphocytes and monocytes has been shown by the end of the 10-day-long
phototherapeutic course. The regulatory character of the single and course sessions of the VIP light on the blood
immunocompetent cells is substantiated: depending on the initial state of the immune system, the VIP light can
produce both stimulating and inhibitory effect on lymphoid cell subpopulations, which opens large possibilities
of using this method for correction of immunological disturbances in diseases of different etiopathogenesis.
Key words: visible and infrared polarized light, mononuclear membrane markers, phototherapy
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