DISTRIBUTION OF CHLAMYDIA AND(OR) CHLAMYDIA-LIKE MICROORGANISMS REVEALED BY LIGHT MICROSCOPE
ANALISYS CONFIRMED BY ELECTRON MICROSCOPE DATA AND FISH HYBRIDIZATION
L. P. Zakharenko,1 * N. B. Rubtsov,1 T. V. Karamysheva,1
E. I. Ryabchikova,2 E. Yu. Bunkova,3 N. N. Kolodina,4 T. I. Nepomnyashchaya 5
1 Institute of Cytology and Genetics, Siberian Branch of the RAS, Novosibirsk,
2 State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk Region,
3 Spa "Belokuricha", Altay Region,
4 Regional Military Hospital, Novosibirsk, and
5 Municipal Hospital, Berdsk, Novosibirsk Region, Russia;
* e-mail: zakharlp@bionet.nsc.ru
Using light microscopy, we have shown that chlamydia and/or chlamydia-like microorganisms are registered in 20-25 %
of the healthy part of human population, whereas in patients of the same age with gynecological problems these were found in 40-50 %.
Commonly, the infection was slightly manifested (less than 5 % of cells are infected). These results were confirmed in four months but
only in heavily infected patients. The light microscope data are confirmed by observations with electron microscopy, and by FISH hybridization
of the total chlamydial DNA on cytological preparations with chlamydial inclusions. In some cases, microcolonies revealed by FISH
hybridization occupied the majority of the cytoplasm volume. Occasionally, the DNA material was found on the nuclear surface. It seems
likely that in heavily infected cells chlamydia are able to penetrate into the perinucular space.
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