ULTRASTRUCTURE OF MITOCHONDRIA IN CARDIOMYOCYTES AFTER INDUCTION OF APOPTOSIS UNDER
ANOXIC CONDITIONS
V. B. Saprunova, L. E. Bakeeva, L. S. Yagujinsky
The A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia;
e-mail: bakeeva@imec.msu.ru
Apoptosis in cardiomyocytes was induced by incubation of pieces of cardiac tissues under condition of anoxia.
Electronmicroscopic investigation detected previously unknown changes in mitochondrial ultrastructure. The mitochondrial population
was characterized by morphological heterogeneity. In addition to a mitochondrial population characterized with irrigated cleared matrix,
anoxia induced the appearance of an atypical and previously unknown population of small electron-dense cardiomyocyte mitochondria.
They were characterized by unusual localization inside electron-light mitochondria ("mitochondria inside mitochondria"). The most part
of mitochondria with the irrigated matrix are commonly characterized by unusual types of re-arrangements of the inner mitochondrial
membrane. Under anoxic conditions, the inner mitochondrial membrane formed electron-dense ordered structures. This is a spongy
structure with cells of equal size. Results of our study are discussed in terms of conception of changes in mitochondrial reticulum
ultrastructure during apoptosis.
Key words: mitochondria, electron microscopy, apoptosis, anoxia
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