ELEVATED EXPRESSION OF ARGENTOFILIC PROTEINS FROM THE NUCLEOLAR ORGANIZER REGIONS IN
MYOCARDIUM OF PATIENTS WITH OBSTRUCTIVE HYPERTROPHIC CARDIOMYOPATHY, AND MUTATIONS IN P53 TUMOR SUPRESSOR
GENE
A. Ya. Gudkova,1 E. V. Shlyakhto,1 N. N. Mamaev,2
M. G. Rybakova,1 Kh. K. Amineva,1 E. N. Semernin,1
K. V. Korzhenevskaya,1 M. I. Zaraisky3
1 Chair of Internal Medicine after G. F. Lang, Pavlov St. Petersburg State Medical University,
2 Chair of Pathological Anatomy, Surgut State University, and
3 St. Petersburg Branch of European Centre of Bone Marrow Transplantation;
Silver staining for nucleolar proteins was used to evaluate the ribosomal genes activity in cardiomyocy-tes (Cm) and
fibroblast-like cells (FBS) of intraventricular septum, and other regions of the left ventricle. Specimens to be analysed were taken from 7
patients with idiopathic obstructive hypertrophic cardiomyopathy (OHCM). In this group the quantity of nucleoli and AgNORs, reflecting the
transcriptional and processsing level of pre-ribosomal RNA in all type of cells, was at least 2 and 3 times higher than in patients with
essential hypertension and in healthy control, respectively. We suggest that nucleolar hypertrophy in patients with hypertrophic
cardiomyopathies, inappropriate to hypertrophy in other pathological conditions, may be most probably of compensatory character,
and this may be in part explained by nuclear hyperploidy and chromosomal endoreduplication. We have noted a marked heterogeneity in
shape, size and quantity of nucleoli, and in AgNORs of FBC. Nuclei with modulated phynotype containing nucleoli of high activity were
revealed. This article presents the first data on p53 mutation indentification in patients with advanced OHCM.
Back
Contents
Main
|
