INHIBITION OF RAT LIVER MITOCHONDRIAL MONOAMINE OXIDASE ACTIVITY BY ALKALOIDS ISOLATED FROM
CHELIDONIUM MAJUS AND MACLEAYA, AND BY DERIVATIVE DRUGS "UKRAIN" AND "SANGUIRYTHRINE"
O. V. Yagodina,1 E. B. Nikolskaya,1
M. D. Faddejeva 2
1 The I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry RAS,
and 2 Institute of Cytology RAS, St. Petersburg, Russia;
2 e-mail: fad@mail.cytspb.rssi.ru
It has been shown that the major alkaloids from plants Chelidonium majus L. and Macleaya (Bocconia) cordata
and microcarpa, namely, berberine, sanguinarine, chelidonine, and drugs "Ukrain" (thiophosphoric acid derivative of a sum of the alkaloids
isolated from Ch. majus L.) and "Sanguirythrine" (a mixture of the alkaloids sanguinarine and chelerythrine, w/w 3 :7, isolated from
Macleaya), are irreversible inhibitors of oxidative deamination reaction of serotonin and tyramine as substrates, catalyzed by rat liver
mitochondrial monoamine oxidase (MAO). At the same time these substances do not influence the oxidative deamination reaction of
benzylamine as substrate (in concentration 1 mM or less). The substrate specificity of this inhibi-tion manifests that mainly the oxidative
deamination reactions catalyzed by MAO form A are inhibited by the agents studied. Among the examined agents, alkaloid chelidonine
and drug "Ukrain" are the strongest in-hibitors of the reaction. Alkaloids berberine and sanguinarine and drug "Sanguirythrine" exhibit a
weaker action. Judging from the data obtained, sanguinarine and chelerythrine appear to exert similar inhibitory ef-fects in this reaction,
since sanguinarine and "Sanguirythrine" have similar values of bimolecular rate cons-tants of their interaction with mitochondrial MAO.
As it is well known, the MAO inhibitors appear to be, as a rule, pronounced antidepressants. The combination of malignotoxicity and
antidepressive activity in drug "Ukrain" seems to be favourable for its clinical applications.
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