THE INFLUENCE OF LAMININ ON STRUCTURAL KARYOTYPIC VARIABILITY OF RAT KANGAROO KIDNEY
CELL LINES
G. G. Poljanskaya, T. S. Goryachaya, G. P. Pinaev
Institute of Cytology RAS, St. Petersburg, Russia;
e-mail: poljansk@mail.cytspb.rssi.ru
The structural karyotypic variability has been investigated in the "markerless" epithelial-like Rat kangaroo kidney cell lines
NBL-3-17 and NBL-3-11 on cultivation on a laminin-2/4 coated surface. In cell line NBL-3-17, cultivated on the laminin-coated surface
for 2, 4 and 12 days, and in cell line NBL-3-11, cultivated on the laminin-coated surface for 2 and 4 days, there is a significant increase
in the frequency of chromosomal aberrations, both chromosomal breaks and dicentrics (telomeric associations). Different sensitivity
of individual chromosomes to inducing chromosomal breaks was observed in addition to a preferential involment of some chromosomes
in dicentric formation. Structural instability of chromosomes at cultivation on laminin demonstrates nonspecific reaction of the "markerless"
cell lines to unfavourable factors of the environment. We discuss possible reasons of differences in the character of karyotypic variability
between a cell line of the Indian muntjac skin fibroblasts and epithelial-like Rat kangaroo kidney cell lines cultivated on laminin.
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