THE INFLUENCE OF LAMININ ON NUMERICAL KARYOTYPIC VARIABILITY OF TWO RAT KANGAROO KIDNEY
CELL LINES
G. G. Poljanskaya, T. S. Goryachaya, N. A. Mikhailova, G. P. Pinaev
Institute of Cytology RAS, St. Petersburg, Russia;
e-mail: poljansk@mail.cytspb.rssi.ru
The numerical karyotypic variability has been investigated in "markerless" epithelial-like Rat kangaroo kidney cell lines
NBL-3-11 and NBL-3-17 on cultivation on a laminin-2/4 coated surface. In cell line NBL-3-17, cultivated on the laminin-coated surface
for 2, 4 and 12 days, the character of numerical karyotypic variability has changed. In 2 days the general character of cell distribution for
the chromosome number did not change, but the frequency of cells with modal number of chromosomes decreases significantly, while
that of cells with lower chromosome number show a tendency to increase. At a prolongation of cultivation time to 4 and 12 days, the
numerical karyotypic heterogeneity in cell population increases due to a significant change in the general character of cell distribution
for the chromosome number, which is caused by a significant decrease in the frequency of cells with the modal number of chromosomes,
and by an increase in the frequency of cells with lower chromosome number. The analysis of distribution of individual chromosomes
showed that the number of types of additional structural variants of the karyotype (SVK) increases significantly on cultivation on laminin
for 2-12 days. In cell line NBL-3-11, cultivated on the laminin-coated surface for 2 and 4 days, the character of numerical karyotypic
variability did not change compared to cont-rol variants. Possible reasons of the observed changes of numerical karyotypic variability in
cell line NBL-3-17 is discussed. The reason of differences in the character of numerical karyotypic variability between cell lines NBL-3-11
and NBL-3-17 possibly consists in the change of gene expression, namely in a dose of certain functioning genes. The polymerase chain
reaction with arbitrary primers revealed no differences between DNA patterns of cell lines NBL-3-17 and NBL-3-11. This can reflect a
similarity in the primary DNA structure of both cell lines. Hence, these lines differ only in the number of homologous chromosomes
(hypotriploid and hypodiploid).
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