ÒHE INFLUENCE OF BURN FLUID ON FUNCTIONAL ACTIVITY
OF Balb/ÇÒÇ CELLS AND THE ROLE OF MATRIX METALLOPROTEINASES IN THIS PROCESS
I. V. Voronkina,1 N. S. Nikolaenko,1 B. A. Paramonov,2
M. I. Blinova,1 G. P. Pinaev 1
1 Institute of Cytology, RAS, and Faculty of Thermal Injuries,
2 Military Medical Academy, St. Petersburg;
e-mail: voron@mail.cytspb.rssi.ru
The influence îf burn fluid and its matrix metalloproteinases (MMPs) on Balb/ÇÒÇ cells was studied. The influence
of burn fluid was assessed by morphology and specific functional activities of cells characteristic of the healing
process - proliferation, monolayer contraction and migration of cells in wound model. The presence of burn fluid in
cultivating medium accelerated cell proliferation by 2.5 times compared to normal conditions, promoted fibroblast
monolayer contraction, and accelerated cell migration on the wound surface, thus stimulating cell functions
necessary for successful heating. This effect is partly due to MMPs. The burn fluid contains, presumably, some
additional factors not inhibited by specific MMP inhibitors EDTA and 1,10-phenantrolin. These factors may stimulate
migration and proliferation of cells. The presence of 1-2 % burn fluid is sufficient for enhancing cell
proliferation.
Key words: matrix metalloproteinases, burn fluid, proliferation, migration, fibroblasts, Balb/ÇÒÇ
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