THE INFLUENCE OF IMMUNOSUPPRESSORS, CYCLOPHOSPHANE AND 5-FLUOROURACYL, UPON NATURAL
CYTOTOXICITY AND ACTIVITY OF LYSOSOMAL ENZYMES IN C3HA MICE SPLENOCYTES
O. N. Pogodina, A. G. Bulychev
Institute of Cytology RAS, St. Petersburg, Russia
Effects of two immunosuppressors, cyclophosphane and 5-fluorouracyl, used in clinical practice for treatment of
oncological diseases, were assessed in respect to cytotoxicity and activity of several lysosomal enzymes located in
splenocyte granules of C3HA mice. 48 h after a single intraperitoneal injection, both preparations produced a marked
lecrease in their cytotoxic activity, which was accompanied by a pronounced splenopathy. Both preparations were
shown to decrease activity of arylsulfatase. Administration of cyclophosphane brought about the rise of activity of
acid lipase as compared to control. Activities of acid phosphatase, α-annosidase, β-galactosidase, and
N-acetyl-β-D-glucosidase did not change after administration of the used immunosuppressors. It may be suggested
that only arylsulfatase and acid lipase are involved in performance and(or) manifestation of the natural killer
activity in splenocytes of the C3HA mice after their administration with cyclophosphane or 5-fluorouracyl.
Key words: cytotoxicity, lysosomal enzymes, splenocyte granules, ÑÇÍÀ mice cyclophosphane or
5-fluorouracyl
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