REGULATION OF hpl-bp74 GENE UNDER THE INFLUENCE OF 1,25-DIHYDROXIVITAMIN
D3 AND 17β-ESTRADIOL IN BREAST ADENOCARCINOMA CELL LINE
N. L. Aksenov,1, * V. M. Mikhelson,1 B. I. Polozhintsev,2
I. M. Spivak,1 P. Tuohima,3 T. Ylikomi 3, 4
1 Institute of Cytology RAS, 194064, St. Petersburg, Russia, 2 St. Petersburg State
Technical University, St. Petersburg, Russia, 3 Tampere University Medical School, Òamðåãå, Finland, and
4 Department of Clinical Chemistry, University Hospital, Tampere, Finland;
* e-mail: NiLAksenov@mail.ru
Data are provided on the upregulation of heterochromatin protein binding protein 74 gene (hpl-bp74) at
RNA transcription level under the influence of stimulation of 1,25-dihydroxyvitamin D3. We also showed
the absence of action of 17β-estradiol on the transcription level of hpl-bp74 breast adencarcinoma cell
line (MCF7). The protein encoded by gene hpl-bp74 can be involved in generalized transcriptional regulation
and thus take part in inhibition of proliferation by 1,25(ÎÍ)2D3. Computer analyses of DNA
sequences of hpl-bp74 enabled us to identify some potential hormone responsive elements responsible for the
binding of nuclear receptor for vitamin D with the DNA motifs.
Key words: heterochromatin protein binding, 1,25-dihydroxyvitamin D3, breast
adencarcinoma cell line (MCF cells)
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